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Image Search Results
Journal: Frontiers in plant science
Article Title: Identification and Phenotypic Characterization of ZEBRA LEAF16 Encoding a β-Hydroxyacyl-ACP Dehydratase in Rice.
doi: 10.3389/fpls.2018.00782
Figure Lengend Snippet: FIGURE 7 | Heterologous expression of recombinant ZL16 proteins in E. coli. (A) Expression of wild-type (WT) and zl16 recombinant proteins in E. coli. –/+ denote before and after adding 1 mM isopropyl-beta-D-thiogalactopyranoside (IPTG) for protein induction. M means molecular weight marker. Arrow indicates the target protein from ZL16 or zl16 (29 kDa) fusing with glutathione S-transferase (26 kDa). (B) Comparison of β-hydroxyacyl-ACP dehydratase (HAD) enzyme activities between the wild type (WT) and zl16 after purification of the recombinant proteins. Data are means ± SD of six replicates. ∗∗P < 0.01.
Article Snippet: The protein was extracted from the resulting supernatant and purified by a
Techniques: Expressing, Recombinant, Molecular Weight, Marker, Comparison